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| Marie, Dominique; Rigaut-jalabert, Fabienne; Vaulot, Daniel. |
| Preservation of cells, choice of fixative, storage, and thawing conditions are recurrent issues for the analysis of phytoplankton by flow cytometry. We examined the effects of addition of the surfactant Pluronic F68 to glutaraldehyde-fixed photosynthetic organisms in cultures and natural samples. In particular, we examined cell losses and modifications of side scatter (a proxy of cell size) and fluorescence of natural pigments. We found that different marine phytoplankton species react differently to the action of Pluronic F68. In particular, photosynthetic prokaryotes are less sensitive than eukaryotes. Observed cell losses may result from cell lysis or from cell adhesion to the walls of plastic tubes that are commonly used for flow cytometry analysis.... |
| Tipo: Text |
Palavras-chave: Phytoplankton; Flow cytometry; Preservation; Pluronic F68; Glutaraldehyde. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00374/48493/48858.pdf |
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| Ramond, Pierre; Sourisseau, Marc; Simon, Nathalie; Romac, Sarah; Schmitt, Sophie; Rigaut-jalabert, Fabienne; Henry, Nicolas; De Vargas, Colomban; Siano, Raffaele. |
| The study of protistan functional diversity is crucial to understand the dynamics of oceanic ecological processes. We combined the metabarcoding data of various coastal ecosystems and a newly developed trait‐based approach to study the link between taxonomic and functional diversity across marine protistan communities of different size‐classes. Environmental DNA was extracted and the V4 18S rDNA genomic region was amplified and sequenced. In parallel, we tried to annotate the Operational Taxonomic Units (OTUs) from our metabarcoding dataset to 30 biological traits using published and accessible information on protists. We then developed a method to study trait correlations across protists (i.e. trade‐offs) in order to build the best functional groups.... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00478/58964/61554.pdf |
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| Hummel, Herman; Van Avesaath, Pim; Wijnhoven, Sander; Kleine-schaars, Loran; Degraer, Steven; Kerckhof, Francis; Bojanic, Natalia; Skejic, Sanda; Vidjak, Olja; Rousou, Maria; Orav-kotta, Helen; Kotta, Jonne; Jourde, Jerome; Pedrotti, Maria Luiza; Leclerc, Jean-charles; Simon, Nathalie; Rigaut-jalabert, Fabienne; Bachelet, Guy; Lavesque, Nicolas; Arvanitidis, Christos; Pavloudi, Christina; Faulwetter, Sarah; Crowe, Tasman; Coughlan, Jennifer; Benedetti-cecchi, Lisandro; Dal Bello, Martina; Magni, Paolo; Como, Serena; Coppa, Stefania; Ikauniece, Anda; Ruginis, Tomas; Jankowska, Emilia; Weslawski, Jan Marcin; Warzocha, Jan; Gromisz, Slawomira; Witalis, Bartosz; Silva, Teresa; Ribeiro, Pedro; Fernandes De Matos, Valentina Kirienko; Sousa-pinto, Isabel; Veiga, Puri; Troncoso, Jesus; Guinda, Xabier; Juanes De La Pena, Jose Antonio; Puente, Araceli; Espinosa, Free; Perez-ruzafa, Angel; Frost, Matt; Mcneill, Caroline Louise; Peleg, Ohad; Rilov, Gil. |
| Within the COST action EMBOS (European Marine Biodiversity Observatory System) the degree and variation of the diversity and densities of soft-bottom communities from the lower intertidal or the shallow subtidal was measured at 28 marine sites along the European coastline (Baltic, Atlantic, Mediterranean) using jointly agreed and harmonized protocols, tools and indicators. The hypothesis tested was that the diversity for all taxonomic groups would decrease with increasing latitude. The EMBOS system delivered accurate and comparable data on the diversity and densities of the soft sediment macrozoobenthic community over a large-scale gradient along the European coastline. In contrast to general biogeographic theory, species diversity showed no linear... |
| Tipo: Text |
Palavras-chave: Soft sediment; Benthos; Species diversity; Densities; Harmonization of methods; European cline; Biogeography; Latitudinal diversity gradient (LDG). |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00376/48675/49010.pdf |
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